Hierarchical organization of DNA-peptide materials guided by secondary structure of collagen like peptides

Project Description

The proposed project explores the structural and mechanical properties of hybrid biomaterials formed through the co-assembly of collagen-like peptides (CLPs) and plasmid DNA (pDNA). These assemblies are driven by multivalent arginine–phosphate interactions, enabling the formation of thermally robust, sheet-like supramolecular structures with long-range order. The study aims to elucidate how peptide rigidity and DNA topology influence hierarchical organization, sheet thickness, and growth mechanisms. Advanced characterization techniques available at the BioPACIFIC MIP facility—including SAXS/WAXS and MicroED, will be employed to correlate molecular structure with macroscopic properties. The results will provide fundamental insights into the design principles of self-assembled, thermally stable biomaterials. This collaboration will also foster student training and research capacity in soft materials science through access to cutting-edge experimental infrastructure.

Instruments