Shimadzu Nexera Hybrid HPLC

Reverse-Phase Analytical/Preparative Hybrid HPLC-MS for the automated purification of peptoids, peptides, oligomers, and small molecules.
UC Santa Barbara
Research Areas
Characterization and Properties
Synthesis and Formulation
Shimadzu Nexera Hybrid HPLC
Shimadzu Nexera Hybrid HPLC

The Shimadzu Nexera Hybrid HPLC system at BioPACIFIC MIP combines analytical chromatography for method development and fraction re-analysis with a preparative HPLC for fraction collection, eliminating the time-consuming tasks involved in moving samples and fractions between normally separate systems. The HPLC system at BioPACIFIC MIP further improves upon the typically UV-based fractionation processes and subsequent off-line mass spectrometry (MS) by inclusion of an inline single quadrupole MS detector. Direct on-line MS information ensures only fractions containing the target compound are collected. Efficiency in scale-up is maximized through use of a single system that can directly identify target compounds, automatically method scout for optimal column and focused gradients, and determine the purity of collected fractions following a purification run.

The Shimadzu Nexera features:

  • Dual prep and analytical flow paths with dedicated UV-Vis and PDA detector;
  • A single quad MS detector with ESI and APCI ionization (2000 m/z max);
  • Automated injection and column switching with matched C4 and C18 analytical and semi-prep columns;
  • Verification of fraction purity with re-injection on the analytical side.


Morgan Bates

Project Scientist
UC Santa Barbara