The Automated Thermal cycler (ATC) is capable of performing PCRs (polymerase chain reactions) at a high-throughput scale. The Thermo Fisher Applied Biosystems Automated Thermal Cycler (ATC) is a compact, robust, and highly integrable PCR instrument designed specifically for automated and high-throughput laboratory workflows. It is engineered to deliver reliable, hands-free PCR amplification, supporting both 96-well and 384-well plate formats, and is optimized for seamless integration with robotic liquid handling systems.

Key Features

Flexible Plate and Volume Compatibility:

• Supports 96-well (0.2 mL) and 384-well (0.02 mL) formats. PCR volume range: 96-well: 10–100 μL for full-skirted plates; 20–100 μL for half-skirted plates 384-well: 5–20 μL for full-skirted plates Compatible with ANSI/SBS-standard plates.

Performance and Temperature Control:

• Thermal range: 0°C to 105°C, supporting a wide variety of PCR protocols. Temperature accuracy: ±0.25°C (35°C to 99.9°C). Temperature uniformity: ≤0.5°C (20 seconds after reaching 95°C). Maximum block ramp rates: 3.5°C/sec (96-well), 2.8°C/sec (384-well); maximum sample ramp rates: 1.8°C/sec (96-well), 1.6°C/sec (384-well).

Automation and Software Integration:

• SiLA Rapid Integration™ standard-compatible software allows straightforward connection to major robotic platforms. Prewritten APIs and free desktop software enable both stand-alone operation and full robotic integration, including simulation modes for legacy Applied Biosystems 9700 systems.

Other features include:

1. Automated lid critical for hands-free operation
2. Automated plate ejectors to ensure consistent plate positioning
3. Heated cover to protect the plate nest, minimizing evaporation and enabling thermal contact