The GFPickolo is a fully automated microbial colony picker. It uses a camera and a program that allows finding, filtering, and sorting colonies based on size, shape, color, isolation distance, and circularity. The GFPickolo is also specifically equiped to pick colonies with the strongest, weakest, average, or largest fluorescent signal. This tool is specifically useful for cloning plasmids containing GFP. It works with petri dishes, multiwell plates, and omnitrays. It also has several picking modes such as scooping, scratching, and multihit, utilizing agar height detection. Inside the Fluent, once the colonies are picked, they can be automatically transferred to a multiwell plate for further experimentation.